RNA

Part:BBa_K1963004:Design

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-07)


An sRNA system for down-regulation of E. coli fliC encoding flagellin.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

This part is RFC[10] compliant.


Source

This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template. A facile PCR-based protocol for the generation of any sRNA system is given on the BBa_K1963002 main page.

References

Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols 8, 1694–1707. doi:10.1038/nprot.2013.105

Erhardt M. (2016) Strategies to Block Bacterial Pathogenesis by Interference with Motility and Chemotaxis. Curr Top Microbiol Immunol [Epub ahead of print] doi:10.1007/82_2016_493

De Maayer P, Cowan DA. (2016) Flashy flagella: flagellin modification is relatively common and highly versatile among the Enterobacteriaceae. BMC Genomics 17:377. doi: 10.1186/s12864-016-2735-x.

Pushkin DO, Bees MA. (2016) Bugs on a Slippery Plane : Understanding the Motility of Microbial Pathogens with Mathematical Modelling. Adv Exp Med Biol 915:193-205. doi: 10.1007/978-3-319-32189-9_12.